IT Calorimetry

The Biophysics Core maintains a Microcal Isothermal Titration Calorimeter to assist in calculating stochiometry, binding constant, enthalpy and entropy. The instrument includes an internal Peltier mechanism. To arrange access, contact Ms. Li Luo [Phone: (860) 679-8404; Email: LiLuo@uchc.edu] or Dr. Jeff Hoch [Phone: (860) 679-3566; Email: Hoch@uchc.edu].

Fees
   Internal users: a small fee (about $2 per hour) to cover maintenance costs.
   External academic users: $8-50 per hour.
   Commercial users: $100 per hour.

http://www.microcalorimetry.com/

ITC References

	Morin, P.E., Freire, E. (1991) Direct calorimetric analysis of the enzymatic activity of yeast cytochrome c oxidase. Biochemistry 30, 8494-8500.
	Sigurskjold, B.W. (2000) Exact analysis of competition ligand binding by displacement isothermal titration calorimetry. Anal. Biochem. 277, 260-266.
	Todd, M.J., Gomez, J. (2001) Enzyme kinetics determined using calorimetry: A general assay for enzyme activity? Anal. Biochem. 296, 179-187.
	Velazquez-Campoy, A., Kiso, Y., Freire, E. (2001) The binding energetics of first- and second-generation HIV-1 protease inhibitors: Implications for drug design. Arch. Biochem. Biophys. 390, 169-175.
	Wiseman, T., Williston, S., Brandts, J.F., Lin, L.-N. (1989) Rapid measurement of binding constants and heats of binding using a new titration calorimeter. Anal. Biochem. 179, 131-137.