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     Biophysical Core

Research Facilities

Research Support

Administration


LC4 from Chlamydomonas reinhardtii binds Ca2+

LC4, a protein associated with dynein in the unicellular alga Chlamydomonas reinhardtii, binds two molecules of Ca2+ when a photoshock induces a change in the direction of flagellar motility.
Lab of Dr. Stephen King

 

  Biophysical Core

The MMSB Biophysical Core is a shared instrument facility equipped with advanced instrumentation for characterizing biological macromolecules. The facility is in the UConn Health Center, L Wing, 3rd floor. The resources of the facility are available to UCHC, other UCONN and external academic investigators at rates given in the fee schedule shown below. Commercial use of the instrumentation can be obtained through special arrangement.

The UCONN Health Center also has state-of-the-art NMR instrumentation for protein structure determination, stability and binding studies through the separate Gregory P. Mullen NMR Structural Biology Facility, http://structuralbiology.uchc.edu.

CONTACT INFORMATION

To gain access to the Biophysical Core Facility, contact any staff member:
Dr. Jeff Hoch, Director
     email: hoch@uchc.edu
     phone: (860) 679-3566

Dr. Mark Maciejewski, Associate Director
     email: markm@neuron.uchc.edu
     phone: (860) 679-1943


BILLING INFORMATION

Invoices will be distributed quarterly. Users from UCHC must indicate FRS coding at the time of use. Users from other UCONN campuses must arrange payment via transfer voucher. External users may pay by check, payable to the UCONN Health Center. External users must provide a written statement from a principal investigator acknowledging and accepting the fee schedule.

Technique Applications Instrument
Circular Dichroism Spectropolarimetry Information on secondary structure: alpha-helix, beta-sheet or random coil
Measure chemical denaturation
Jasco J-715 Spectropolarimeter
http://www.jascoinc.com
Differential Scanning Calorimetry, with robotic sampler Information about subunit composition, folding and thermal stability
Measure binding thermodynamics
    Kd to 10 -20 M
MicroCal VP-Capillary DSC
http://www.microcal.com/index.php?id=19
Isothermal Titration Calorimetry Calculate stoichiometry, binding constant, enthalpy and entropy
    Kd 10-6 to 10 -11 M
MicroCal VP-ITC
http://www.microcal.com/index.php?id=14
Matrix-assisted Laser Desorption Ionization Mass Spectrometry Protein identification
Accurate mass determination
ABI Voyager DE Pro
https://products.appliedbiosystems.com:443/ab/en/US/adirect/ab?cmd=catNavigate2&catID=600852
Multi-angle Laser Light Scattering Detection Molecular weights of the different species in a sample and their size distribution
Determine aggregation state
Wyatt Technology MiniDawn
http://www.lightscattering.com/solutions/hardware/minidawn.cfm
Ultraviolet-Visible Spectrophotometry Measure protein concentration Cary 1E
http://www.varianinc.com/cgi-bin/nav?products/spectr/uv/index&cid=JKQLHOLKFJ
Vapor-pressure Osmometry Measure water activity and total solute concentration