The MMSB Biophysical Core is a shared instrument facility equipped with advanced instrumentation for characterizing biological macromolecules. The facility is in the UConn Health Center, L Wing, 3rd floor. The resources
of the facility are available to UCHC, other UCONN and external academic investigators at rates given in the fee schedule shown below. Commercial use of the instrumentation can be obtained through special arrangement.
The UCONN Health Center also has state-of-the-art NMR instrumentation for protein structure determination, stability and binding studies through the separate Gregory P. Mullen NMR Structural Biology Facility, http://structuralbiology.uchc.edu.
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CONTACT INFORMATION
To gain access to the Biophysical Core Facility, contact any staff member:
Dr. Jeff Hoch, Director
email: hoch@uchc.edu
phone: (860) 679-3566
Dr. Mark Maciejewski, Associate Director
email: markm@neuron.uchc.edu
phone: (860) 679-1943
Ms. Li Luo, Research Associate
email: li@uchc.edu
phone: (860) 679-2998
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BILLING INFORMATION
Invoices will be distributed quarterly. Users from UCHC must indicate FRS coding at the time of use. Users from other UCONN campuses must arrange payment via transfer voucher.
External users may pay by check, payable to the UCONN Health Center. External users must provide a written statement from a principal investigator acknowledging and accepting the fee schedule.
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| Technique |
Applications |
Instrument |
| Circular Dichroism Spectropolarimetry |
Information on secondary structure: alpha-helix, beta-sheet or random coil
Measure chemical denaturation |
Jasco J-715 Spectropolarimeter
http://www.jascoinc.com |
| Differential Scanning Calorimetry, with robotic sampler |
Information about subunit composition, folding and thermal stability
Measure binding thermodynamics
Kd to 10 -20 M |
MicroCal VP-Capillary DSC
http://www.microcal.com/index.php?id=19 |
| Isothermal Titration Calorimetry |
Calculate stoichiometry, binding constant, enthalpy and entropy
Kd 10-6 to 10 -11 M |
MicroCal VP-ITC
http://www.microcal.com/index.php?id=14 |
| Matrix-assisted Laser Desorption Ionization Mass Spectrometry |
Protein identification
Accurate mass determination |
ABI Voyager DE Pro
https://products.appliedbiosystems.com:443/ab/en/US/adirect/ab?cmd=catNavigate2&catID=600852 |
| Multi-angle Laser Light Scattering Detection |
Molecular weights of the different species in a sample and their size distribution
Determine aggregation state |
Wyatt Technology MiniDawn
http://www.lightscattering.com/solutions/hardware/minidawn.cfm |
| Ultraviolet-Visible Spectrophotometry |
Measure protein concentration |
Cary 1E
http://www.varianinc.com/cgi-bin/nav?products/spectr/uv/index&cid=JKQLHOLKFJ |
| Vapor-pressure Osmometry |
Measure water activity and total solute concentration |
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©2006, 2007 UCHC, Department of Molecular, Microbial and Structural Biology